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CellDETA™ Products

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Applications 
Ideal product for intactly harvesting cells, especially for fragile cells such as stem cells and neuron cells, and macrophage cells etc., and also for tissue culture, extra cellular matrix research, and so on. 
For more iormation,please click on: http://www.jetbiofil.com/en/about.html?introID=44604

Product Specifications And Details

Temperature-Responsive Cell Culture Products 
Thank you for using a product with temperature-responsive JET CellDETA culture surface. The temperature-sensitive polymer with thickness of 15-20 nanometer chemically covalently immobilized on the culture surface. Cells can be spontaneously intactly detached from JET CellDETAculture surface under 20-25°C without enzymatic processing which is well known for its disfunction of cells. Keeping their activities is possible with JET CellDETA culture surface for cell culture. 



Cautions 
   Please ensure that there is no damage to the package and read the following instructions carefully to ensure the maximum performance of this product. 
   The product is intended for research purposes only. Any other use is not warranted by Jet coporation. Do not use the product for clinical or diagnostic purposes. Products with JET CellDETA surface are intended for single-use only. 
   Do not re-use the product as cell secreted and medium-derived components may be adsorbed to the surface after cell harvest, compromising performance in repeated use. 
   Re-sterilization and scratching of the culture surface may compromise the JET CellDETA surface properties. 

In vitro cell culture 
The temperature-responsive polymer chemically covalently grafted to the culture surface area becomes slightly hydrophilic above 32°C, allowing cells to adhere. So it is recommended that cells are preferable incubated at 37°C. 

The JET CellDETA surface becomes hydrophobic below 32°C, causing cell detachment from the surface. 
To avoid undesired cell detachment during seeding cells and medium changes, pre-warming culture surface and culture medium (37°C) to facilitate cell attachment is suggested. Observations under a microscope should also be kept short in order to prevent cooling and undesired cell detachment. Seed cells evenly over the culture area to avoid uneven distribution of the cells in culture. 
Attachment time for cells may vary with cell type. 
 Should you have difficulty in attaching cells, pre-coating of the culture surface with collagen, fibronectin, polylysine, laminin or the use of medium with serum may ease cell attachment. 

Cells harvest 
Culture cells on the JET CellDETA surface to a density before confluence, that is cell-cell junctions are not established, cells harvest are readily conducted by slight agitation and pipetting. 
Place the product with JET CellDETAsurface in an environment set at 20-25°C in order to detach the cells. Alternatively, the product may be placed at room temperature (20–25°C) under sterile conditions. For quicker cell detachment, the culture medium can be exchanged to 20–25°C culture medium prior to detachment. 
Cells should detach spontaneously within 20-60 minutes, some cells may not detach completely, depending on the cell type and detachment conditions. 
Cells that fail to detach spontaneously can be detached by gentle plate/dish agitation, or by gently flushing the surface with medium. 

Storage 
The product should be stored at room temperature (20–35°C) and out of direct sunlight. 

Disposal 
Ensure the used product without biological and chemical pollution to the environment and disposed as recyclable plastics. 
The product is prepared and shipped under high quality control. However, should you have any claims or questions, please contact us for free. 







Figure1.(a)Schematic representaion of the adhesion(37 °C)and detachment(20°C)of a cell on a poly(N-isopropylamide)-grafted surface. Cell on PNIPAM-coated dishes at 37°C and 20°C. Note the elongated shape of cells on the left micrograph characteristic of a supported cell cultur, and rounded cell shape on the right characterstic.

CellDETA Dish

Cat. No.

Diameter

(mm)

Surface Type

Sterile

Cell Growth Area

(cm2)

Qty.Per 

bag/case

CDD022100

100

 CellDETA

Surface Treated

Y

60.8

10/240


CellDETA Flask

Cat. No.

Volume

(ml)

Surface Type

Sterile

Cell Growth Area

(cm2)

Qty.Per 

bag/case

CDF022250

250

CellDETA

Surface Treated

Y

75.0

5/100


 


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