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Custom sgRNA

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Synthetic single guide (sgRNA) for efficient genome editing

Pure and simple

CRISPR application requires 2 key functional elements: target-specific guide RNA and CAS9 enzyme. Scientists have created gRNA using multiple methods, such as transfection of gRNA-expression plasmids, in vitro transcription, or by annealing a short crRNA oligo with a tracrRNA scaffold.

Recently, synthetic single guide has been recognized as the preferred way for highly efficient and accurate editing. The synthetic single gRNA is a pure 100-mer RNA oligo that contains the target gRNA sequence and the tracrRNA scaffold in a single entity.

Benefits of synthetic sgRNA (100mer):

  • Simple workflow
    Ready-to-use solution
    No in vitro transcription (as in IVT)
    No annealing/purification (as in the 2-oligo system)
  • Better in vivo stability
    No worry for RNase carry-over from the source
  • 100% DNA-free
    No risk of integrating foreign DNA into cell line
  • Better efficiency
    Up to 90% genome editing efficiency
  • Cost-effective
    Highly scalable for large numbers of experiments
  • Fast delivery
    sgRNA shipped out in 5-7 days

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The editing efficiency (solid bars) and consistency (error lines) of synthetic sgRNAs against in vitro transcribed (IVT) guide RNAs in HEK293T cells. The experiment was conducted by a third-party using three different gene targets and was replicated three times.


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