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首頁 經銷廠牌 GenLab Taq DNA Polymerase 1000U

GenLab Taq DNA Polymerase 1000U

Genlab Taq DNA Polymerase is ideal for routine PCR applications

 Description

Taq DNA polymerase is a recombinant 94 kDa DNA polymerase obtained by expression of the modified Thermus aquaticus DNA polymerase gene in E. coli.

This thermostable enzyme exhibits a strong 5’ to 3’ polymerase activity and generates with 3’ dA overhang but has no 3’ to 5’ exonuclease (proofreading) activity.

 

Ordering Information

Genlab Taq DNA polymerase 1,000 units

Supplied with 10X Reaction Buffer (1.25 ml/vial x 3 vials)

 

Concentration

5 units/μl

 

Unit assay conditions

25 mM TAPS, pH 9.3 (at 25°C), 50 mM KCl, 2 mM MgCl2, 1 mM DTT, 0.5 mg/ml activated salmon sperm DNA, 0.2 mM dATP, dCTP, dGTP, dTTP

 

Storage and Dilution Buffer

50 mM Tris-HCl, pH 7.9 (at 25°C), 1 mM EDTA, 5 mM DTT, 100 mM NaCl, 1% Triton X-100, 50% (v/v) glycerol and stabilizers.

10X Reaction Buffer

250 mM KCl, 100 mM Tris-HCl, pH 9.2, 15 mM MgCl2 and 1% Triton X-100.

 

Storage Conditions

Store Taq DNA polymerase at -20°C in a constant freezer.                         

Unit Definition

One unit of enzyme is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 74°C.

 

Example of Reaction Composition

 

 

Volume/Reaction

Final Concentration 1X

10X Reaction Buffer

5 μl

1X

dNTP Mix (2.5 mM)

4 μl

200 μM of each dNTP

Forward Primer (10 μM)

Variable

0.1–0.5 μM

Reverse Primer (10 μM)

Variable

0.1–0.5 μM

DNA Polymerase

(5 U/μl)

0.25 μl

1.25 units/reaction

Distilled water (PCR Grade)

Variable

-

Template DNA

Variable

1 μg/reaction

Total Volume

50 μl

 

Note: If smaller or larger reaction volumes are used, please reduce or   increase the amount of each component accordingly.

 For Research Use Only

 

 

Temp.

Time

Number of Cycle

Initial denaturation

94°C

3

1 cycle

Denaturation

94°C

0.5–1 min

25 to 40 cycles

Annealing

50–68°C

0.5–1 min

Extension

72°C

1 min

Final extension

72°C

10 min

1 cycle

Note: For PCR products longer than 1 kb, use an extension rate at approximately   1 kb/min

 

For Research Use Only

 


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